In the viral genome, two major open reading frames ( ORF1 and ORF2) in the plus strand and one minor ORF ( ORF3) in the complementary strand were identified. VD2基因组正链含有2个大的开放阅读框,负链含有1个小的开放阅读框。
Methods Using digoxin labelled plus strand and minus strand HCV RNA probes, an in situ hybridization technique suitable for detecting HCV RNA in the peripheral blood mononuclear cells ( PBMC) was established. 方法应用地高辛素标记HCVRNA正链及负链探针,建立原位杂交方法检测外周血单个核细胞(PMBC)中的HCVRNA。
In the cancer tissues of a same case, if minus strand RNA of HCV was positive, its signal intension had no significant difference from that of HCV plus strand RNA. 在同例肝(癌)组织中,若负链HCVRNA阳性,则其信号强度与正链基因无明显差别;
The relapse occurred in patients whose plus strand HCV RNA in PBMCs remained positive before and after treatment. 复发见于治疗前后PBMCs中正链HCVRNA持续阳性的患者。
HCV RNA in PBMC was extracted and tested for both plus and minus strand by RT-PCR. All of these serum were anti-HCV and HCV RNA positive. 所选检测对象血清标本抗-HCV及HCVRNA均为阳性。